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Figure 3 | BMC Research Notes

Figure 3

From: Immunofluorescence labeling of cell surface antigens in Dictyostelium

Figure 3

Loss of different surface antigens during permeabilization. Cells were labeled following a classical immunofluorescence procedure (upper panel) or a two-step procedure (lower panel). In both cases, cells were permeabilized with methanol at −20°C. The antibodies used detected csA-SA (A, B), H36 (C, D), p25 (E, F), p23 (G, H) or p80 (I, J). The effect of cell permeabilization on surface labeling differed for the various antigens considered. Scale bar: 5 μm.

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