Figure 4

Thin layer chromatography (tlc) of the hydrolytic products generated by rhhman on Mannans and Manno-oligosaccharides. The reactions in the substrates respectives in 0,1 M sodium citrate buffer (pH 5.5) with 0,4 μg of rHhMan were incubated at 30°C for 24 hours. 2 μl of each reaction were analized by TLC. The substrates were A) Guar gum, B) Locust vean, C) Manno- oligosaccharides: mannobiose, mannotriose and mannotetraose and D) Manno-oligosaccharides: Mannopentose. The incubation time was shown (minutes and hours). The standar was a mix of mannose and manno- oligosaccharides where M1 is mannose; M2, mannobiose; M3, mannotriose; M4, mannotetraose y M5 mannopentose.