Table 1 Guinea pig reference gene primer validation using liver cDNA
Gene | Slope | Amplification efficiency (%) | Correlation coefficient |
|---|---|---|---|
ACTB | −3.4077 | 96.5 | 0.9996 |
ATP5B | −3.3395 | 99.3 | 0.9997 |
ATP6 | −3.4453 | 95.1 | 0.9996 |
B2M | −3.2484 | 103.2 | 0.9975 |
CFL1 | −3.2969 | 101.1 | 0.9983 |
CLTC | −3.3034 | 100.8 | 0.9973 |
CTBP1 | −3.2946 | 101.2 | 0.9998 |
GAPDH | −3.3797 | 97.6 | 0.9997 |
HMBS | −3.2421 | 103.4 | 0.9962 |
PPIB | −3.2028 | 105.2 | 0.9981 |
RPLP0 | −3.3246 | 99.9 | 0.9984 |
SDHA | −3.3581 | 98.5 | 0.9999 |
TBP | −3.2961 | 101.1 | 0.9979 |
TFRC | −3.3674 | 98.1 | 0.9993 |
TPT1 | −3.2627 | 102.5 | 0.9980 |