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Table 2 Reference genes ranking for the entire dataset calculated by the GeNorm, NormFinder and BestKeeper

From: Validation of reference genes for normalization of qPCR gene expression data from Coffea spp. hypocotyls inoculated with Colletotrichum kahawae

Entire dataset
Gene GeNorm Normfinder Bestkeeper
M SV CV ± SD r
IDE 0.578(1/2) 0.402(1) 2.54 ± 0.62(2) 0.801*
14-3-3 0.578(1/2) 0.624(4) 2.61 ± 0.59(4) 0.723*
RPL7 0.604(3) 0.542(3) 1.98 ± 0.47(1) 0.701*
β-Tub9 0.684(4) 0.478(2) 2.44 ± 0.47(3) 0.720*
S24 0.731(5) 0.610(5) 2.79 ± 0.57(5) 0.660*
UBQ9 0.785(6) 0.699(6) 2.23 ± 0.54(6) 0.560
GAPDH 1.019(7) 1.597(7) n.a n.a
  1. SV. stability value; CV, coefficient of variance; SD, standard deviation of Cq value; r. Pearson coefficient of correlation; * p ≤ 0.001. p-value associated with the Pearson coefficient of correlation. Ranking order is presented in parenthesis, n.a., not assigned.