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Table 3 Reference genes ranking for Catimor 88 and Caturra inoculated with C. kahawae

From: Validation of reference genes for normalization of qPCR gene expression data from Coffea spp. hypocotyls inoculated with Colletotrichum kahawae

Biotic stress Catimor 88 (resistant) Caturra (susceptible)
Gene GeNorm NormFinder BestKeeper GeNorm NormFinder BestKeeper
M SV CV ± SD r M SV CV ± SD r
IDE 0.640(1) 0.505(2) 2.82 ± 0.69(4) 0.790* 0.392(1/2) 0.358(3) 2.09 ± 0.50(2) 0.786*
14-3-3 0.669(2) 0.759(4) 2.59 ± 0.58(3) 0.689* 0.518(5) 0.517(5) 2.71 ± 0.61(5) 0.778*
RPL7 0.640(3) 0.626(3) 2.09 ± 0.49(1) 0.619* 0.502(4) 0.455(4) 1.86 ± 0.44(1) 0.684*
β-Tub9 0.795(4) 0.479(1) 2.34 ± 0.45(2) 0.726* 0.392(1/2) 0.322(1) 2.37 ± 0.46(4) 0.822*
S24 0.914(5) 0.807(6) 2.87 ± 0.59(5) 0.543 0.476(3) 0.341(2) 2.34 ± 0.48(3) 0.787*
UBQ9 0.870(6) 0.735(5) 1.75 ± 0.43(6) 0.303 0.648(7) 0.649(7) 2.61 ± 0.63(6) 0.642*
GAPDH 1.169(7) 1.779(7) n.a n.a 0.600(6) 0.580(6) 2.85 ± 0.58(7) 0.778*
  1. SV. stability value; CV, coefficient of variance; SD, standard deviation of Cq value; r. Pearson coefficient of correlation; * p ≤ 0.05, p-value associated with the Pearson coefficient of correlation Ranking order is presented in parenthesis, n.a., not assigned.