Selection of reference genes from two leafhopper species challenged by phytoplasma infection, for gene expression studies by RT-qPCR

Table 1 Details of the primer pairs used for qPCR

Target gene	Primer name	5′-3′sequence	Amplicon size (bp)	Annealing Temp.	Melting peak	PCR Efficiency	R²
Ribosomal 18S	Mq Fw	AACGGCTACCACATCCAAGG	98	65°C	81.5°C^§ 82.2°C^†	99%	0.982
Ribosomal 18S	Mq Rv2	GCCTCGGATGAGTCCCG	98	65°C	81.5°C^§ 82.2°C^†	99%	0.982
Actin	ActFw832	AAGGACCTGTACGCCAACAC	190	65°C	83°C^§†	83%	0.987
Actin	ActRv1021	GCTGGAAGGTGGACAGAGAG	190	65°C	83°C^§†	83%	0.987
ATP synthase β	ATPβFw622	CGCTTTACTCAGGCTGGTTC	171	60°C	84.5°C^§ 83.5°C^†	100%	0.995
ATP synthase β	ATPβRv792	GTCATCAGCTGGCACGTAGA	171	60°C	84.5°C^§ 83.5°C^†	100%	0.995
GAPDH	GAPFw632	ATCCGTCGTCGACCTTACTG	51	60°C	77.8°C^§ 77.5°C^†	99%	0.998
GAPDH	GAPRv682	TCATCGTAGCTGGCTTCCTTG	51	60°C	77.8°C^§ 77.5°C^†	99%	0.998
Tropo-myosin	TMFw237	AAACGCCGAGAGTGAGGTG	225	60°C	87.1°C^§	85%	0.892
	TMRv461	AAGAACCGAGCCTCCTTCAG	225	60°C	87.1°C^§	85%	0.892
	TMFw33	GAAGCTGGAGAAGGACAACG	143	60°C	85.2°C^†	118%	0.947
	TMRv175	TGTCCAGCTCGTTCTCAATG

ISSN: 1756-0500