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Table 1 Composition of the two media used for studying the effect of acid stress factors on nodulation and growth of soybean

From: Vermiculite’s strong buffer capacity renders it unsuitable for studies of acidity on soybean (Glycine max L.) nodulation and growth

  Chemical B&D nutrient solution Modified Herridge nutrient solution
1 CaCl2 · 2H20 1000 μM 2500 μM
2 KH2P04 500 μM 1000 μM
3 K2HPO4 - 1000 μM
4 Fe citrate 10 μM -
5 Fe(III)-EDTA - 100 μM
6 MgSO4 · 7H20 250 μM 2000 μM
7 K2SO4 1500 μM -
8 KCl - 1500 μM
Micro nutrient   
9 MnSO4 · H20 1 μM -
10 MnCl2 · 2H2O - 11 μM
11 H3BO3 2 μM 46 μM
12 ZnSO4 · 7H20 0.5 μM -
13 ZnCl2 - 0.8 μM
14 CuSO4 · 5H20 0.2 μM -
15 CuCl2 · 2H2O - 0.3 μM
16 CoSO4 · 7H20 0.1 μM -
17 Na2MoO4 · 2H20 0.1 μM 0.1 μM
  1. The desired pH was adjusted using 5 N HCl or 5 N NaOH.
  2. Depends on the treatment, MES buffer of 20 mM was either added or not to the nutrient solutions.