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Table 1 Composition of the two media used for studying the effect of acid stress factors on nodulation and growth of soybean

From: Vermiculite’s strong buffer capacity renders it unsuitable for studies of acidity on soybean (Glycine max L.) nodulation and growth

 

Chemical

B&D nutrient solution

Modified Herridge nutrient solution

1

CaCl2 · 2H20

1000 μM

2500 μM

2

KH2P04

500 μM

1000 μM

3

K2HPO4

-

1000 μM

4

Fe citrate

10 μM

-

5

Fe(III)-EDTA

-

100 μM

6

MgSO4 · 7H20

250 μM

2000 μM

7

K2SO4

1500 μM

-

8

KCl

-

1500 μM

Micro nutrient

  

9

MnSO4 · H20

1 μM

-

10

MnCl2 · 2H2O

-

11 μM

11

H3BO3

2 μM

46 μM

12

ZnSO4 · 7H20

0.5 μM

-

13

ZnCl2

-

0.8 μM

14

CuSO4 · 5H20

0.2 μM

-

15

CuCl2 · 2H2O

-

0.3 μM

16

CoSO4 · 7H20

0.1 μM

-

17

Na2MoO4 · 2H20

0.1 μM

0.1 μM

  1. The desired pH was adjusted using 5 N HCl or 5 N NaOH.
  2. Depends on the treatment, MES buffer of 20 mM was either added or not to the nutrient solutions.