Figure 10From: Nitrogen starvation-induced transcriptome alterations and influence of transcription regulator mutants in Mycobacterium smegmatisAutoregulation of AmtR. Hybridization experiments were carried out with RNA isolated from nitrogen-supplied cells and after different intervals of nitrogen starvation and a probe specific for amtR mRNA. Since the deletion in amtR only comprises the DNA binding domain encoding nucleotides, hybridization of the amtR probe is possible with RNA isolated from strains YL1 (∆amtR) and YL2 (∆glnR∆amtR).Back to article page