Figure 1

Light microscopy of mycelia from A. oryzae NSK before and after cell disruption. (a) Original structure of mycelia before cell disruption with X4 magnification; (b) Original structure of mycelia before cell disruption with X40 magnification; (c) Enzymatic lysis by Yatalase^® (30 min, 3 mg/ml, 30% w/v cell concentration, 60˚C) with X10 magnification; (d) Enzymatic lysis by Yatalase^® with X40 magnification; (e) Cell permeabilized by toluene (0.2% (v/v) toluene, cell concentration 10% (w/v), 120 min, 25°C) with X10 magnification; (f) Cell permeabilized by toluene with X40 magnification; (g) After sonication with X10 magnification (cell concentration 12% (w/v), 8 mL suspension volume, 50 W & 20 min) and (h) After sonication with X40 magnification.