Figure 3

RT-IHC results, using fluorescently labeled primary or secondary antibody . Binding traces produced in LigandTracer, depicting the binding of A) Alexa Fluor 488 labeled anti-mouse antibody after 3 h pre-incubation with anti-RBM3 antibody, B) direct binding of Texas Red-labeled anti-RBM3 antibody at increasing concentrations, added stepwise (6, 12, 21 nM – 80-100 min incubation of each concentration), C) Alexa Fluor 488 labeled anti-mouse antibody after 3 h pre-incubation with anti-SATB2 antibody, D) direct binding of Texas Red-labeled anti-SATB2 antibody at increasing concentrations, added stepwise (6, 12, 21 nM – 80-100 min incubation of each concentration). In A and B, measurements were done in nasopharynx (black solid line), urinary bladder (black dashed line), testis (grey solid line) and tonsil (grey dashed line). Measurements C and D were performed in colon (black solid line), tonsil (black dashed line), liver (grey solid line) and heart muscle (grey dashed line). I = Fluorescent intensity units.