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Table 2 Expression stability index (M-value) and ranking of the candidate reference genes

From: Evaluation of reference genes for reverse transcription quantitative PCR analyses of fish-pathogenic Francisella strains exposed to different growth conditions

    

M-value

    

Strain

ftsz

polA

fopA

ribC

gyrA

uvrD

16S rRNA

rpoB

NCIMB14265 T

0.531

0.818

0.531

0.751

0.949

1.050

2.004

2.563

DSM21254 T

0.555

0.692

0.716

1.009

0.555

0.892

1.496

2.343

PQ 1106

0.603

0.603

0.653

0.842

0.880

1.241

2.570

1.781

All strains combined

0.979

0.790

1.535

0.790

1.498

1.675

2.411

2.126

Ranking order

1

2

3

4

5

6

7

8

  1. The lowest M-value corresponds to the most stable gene.