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Table 10 Alignment statistics of reads mapped against Flaviviridae genomes

From: False positives complicate ancient pathogen identifications using high-throughput shotgun sequencing

Platform

Sample Description

Reads Mapped (% Mapped)

Molecular Length (bp)

Total Mapped (bp)

HeliScope

Grand Plaza Bulk 1

93 (0.00%)

24.6 ± 0.8

2,284

 

Grand Plaza Bulk 2

111 (0.00%)

24.6 ± 0.9

2,726

 

Grand Plaza Phos. 1

112 (0.00%)

24.7 ± 0.9

2,762

 

Grand Plaza Phos. 2

76 (0.00%)

24.8 ± 1.0

1,881

 

Churchyard Bulk 1

122 (0.00%)

24.6 ± 1.3

3,004

 

Churchyard Bulk 2

126 (0.00%)

24.7 ± 1.1

3,116

 

Churchyard Phos. 1

106 (0.00%)

24.5 ± 0.8

2,602

 

Churchyard Phos. 2

105 (0.00%)

24.5 ± 0.8

2,573

 

Grand Plaza Sheared

0 (0.00%)

0

 

Churchyard Sheared

0 (0.00%)

0

 

Soil Bulk

3 (0.00%)

24.3 ± 0.6

73

 

Soil Sheared

0 (0.00%)

0

HiSeq 2500

TP04

0 (0.00%)

0

 

TP09

0 (0.00%)

0

 

TP10

0 (0.00%)

0

 

TP18

0 (0.00%)

0

 

TP37

0 (0.00%)

0

 

TP48

0 (0.00%)

0

 

Soil Bulk

0 (0.00%)

0

  1. “Bulk” samples are the unsheared bulks. “Phos.” samples are the phosphatase-treated unsheared bulks. “Sheared” samples are the sheared bulks. “% Mapped” indicates the percentage of the total number of reads comprised by the reads mapped onto Flaviviridae genomes. “Molecular Length” is the mean length (± standard deviation) of the mapped reads.