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Table 3 Alignment statistics of reads mapped against the human genome

From: False positives complicate ancient pathogen identifications using high-throughput shotgun sequencing

Platform

Sample Description

Reads Mapped (% Mapped)

Molecular Length (bp)

Total Mapped (bp)

HeliScope

Grand Plaza Bulk 1

254,977 (0.82%)

25.2 ± 2.7

6,414,294

 

Grand Plaza Bulk 2

283,309 (0.77%)

25.2 ± 2.8

6,013,180

 

Grand Plaza Phos. 1

186,016 (0.71%)

25.1 ± 2.5

4,668,531

 

Grand Plaza Phos. 2

152,275 (0.66%)

25.2 ± 2.7

3,838,807

 

Churchyard Bulk 1

387,992 (1.34%)

27.3 ± 5.3

10,579,724

 

Churchyard Bulk 2

382,493 (1.41%)

27.3 ± 5.3

10,425,423

 

Churchyard Phos. 1

250,427 (0.88%)

26.2 ± 4.3

6,559,094

 

Churchyard Phos. 2

312,392 (1.05%)

26.5 ± 4.7

8,290,314

 

Grand Plaza Sheared

254 (6.21%)

24.5 ± 1.0

6,226

 

Churchyard Sheared

340 (6.40%)

24.5 ± 0.8

8,325

 

Soil Bulk

9,441 (1.12%)

24.9 ± 2.1

234,694

 

Soil Sheared

2,895 (2.35%)

24.8 ± 1.9

71,816

HiSeq 2500

TP04

2,655 (0.03%)

214.4 ± 39.6

569,144

 

TP09

27,430 (0.41%)

192.1 ± 36.4

5.267,951

 

TP10

1,332 (0.02%)

204.9 ± 37.6

272,903

 

TP18

5,521 (0.00%)

201.7 ± 35.4

1,113,561

 

TP37

6,172 (0.00%)

191.0 ± 31.4

1,179,076

 

TP48

300 (0.00%)

208.6 ± 39.3

62,568

 

Soil Bulk

37 (0.00%)

181.2 ± 34.0

6,706

  1. “Bulk” samples are the unsheared bulks. “Phos.” samples are the phosphatase-treated unsheared bulks. “Sheared” samples are the sheared bulks. “% Mapped” indicates the percentage of the total number of reads comprised by the reads mapped onto the human genome. “Molecular Length” is the mean length (± standard deviation) of the mapped reads.