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Table 1 Purification of β-sitosterol (1) from crude soybean oil extract

From: Rapid purification of gram quantities of β-sitosterol from a commercial phytosterol mixture

Purification step Fraction Stigmasterol β-Sitosterol Campesterol
   (1) (2) (3)
  [% of total phytosterols]
Crude soybean oil extract (S5753, Sigma-Aldrich)
  n.a. 9.3 60.7 27.8
Fractional crystallization
Acetone S 8.5 51.2 40.3
  L 12.3 57.9 29.8
Hexane/toluene/ S 12.3 57.7 30.1
ethanol (4:2:1) L 4.5 66.0 29.5
Diethylether S 12.4 61.5 26.2
  L 3.0 58.1 39.0
Silica column chromatography
  S 7.1 78.2 14.7
  L 1.5 82.0 16.5
Na-Y zeolite chromatography
  S 4.1 92.2 3.7
  L 0.1 94.2 5.7
  1. Values represent averages of replicated experiments (n = 3–5).