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Table 2 NGS read counts and distribution of Cq values

From: A detailed analysis of next generation sequencing reads of microRNA expression in Barrett’s Esophagus: absolute versus relative quantification

NGS reads

Average Cq values

Average delta Cq values

0-10

29.7

8.6

11-100

28.5

9.1

101-1000

29.1

7.7

1001-10000

27.2

6.6

10001-100000

25.3

3.2

>100000

21.1

1.4

  1. NGS, next generation sequencing, NGS reads for a specific miRNA refers to the counts/million miRNA reads.
  2. Cq, threshold value on qRT-PCR.
  3. deltaCq, Cq(miRNA)-Cq(RNU6B).
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BMC Research Notes

ISSN: 1756-0500

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