Figure 2

Assessment of chlamydial inclusions by immunofluorescence. Monocytes were infected by CP or CT in parallel with LLC-MK2 cells. At 24 hours post-infection, the cells were disrupted and the supernatants were used to infect fresh permissive monolayers cells. At 3 days after the inoculum, chlamydial inclusions were observed by fluorescence microscopy (400× magnification) after incubation with fluorescein-conjugated monoclonal antibodies against Chlamydia genus-specific LPS. A: LLC-MK2 inoculated with supernatant of CP-infected permissive cells. B: LLC-MK2 inoculated with supernatant of CT-infected permissive cells. C: LLC-MK2 inoculated with supernatant of CP-infected monocytes. D: LLC-MK2 inoculated with supernatant of CT-infected monocytes.