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Figure 2 | BMC Research Notes

Figure 2

From: Influence of the length of target DNA overhang proximal to the array surface on discrimination of single-base mismatches on a 25-mer oligonucleotide array

Figure 2

Array analysis of PCR products with similar overall length but different 5′ segment lengths: probe set 1. Panels show the signal intensity for perfect match and mismatch probes hybridised to PCR products with total lengths between 293 and 323 bases and 5′ segment lengths ranging from 43 to 272 bases; each panel shows the effect of a different mismatch at three positions within the probe. Approximately 1012 copies of PCR amplicon were applied to each array. Results shown are mean values for duplicate spots on the same array (error bars show standard deviation). 5AA = AA mismatch between probe and PCR product located in the 5′ third of the probe, etc.

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