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Table 2 Ranking of the eight candidate reference genes according to their expression stability in two different algorithms GeNorm and BestKeeper in Barnea, Frantoio and Picual individually and together

From: Validation of reference genes for gene expression analysis in olive (Olea europaea) mesocarp tissue by quantitative real-time RT-PCR

 

Barnea + Frantoio + Picual

Barnea

Frantoio

Picual

Ranking

GeNorm

BestKeeper

GeNorm

BestKeeper

GeNorm

BestKeeper

GeNorm

BestKeeper

1

GAPDH

PP2A

EF1-Alpha

PP2A

EF1-Alpha

GAPDH

60S RBP L18-3

60S RBP L18-3

2

EF1-Alpha

EF1-Alpha

GAPDH

EF1-Alpha

60S RBP L18-3

EF1-Alpha

PP2A

OUB2

3

PP2A

GAPDH

PP2A

GAPDH

PTB

OUB2

OUB2

PP2A

4

60S RBP L18-3

PTB

OUB2

OUB2

OUB2

60S RBP L18-3

EF1-Alpha

EF1-Alpha

5

PTB

60S RBP L18-3

TUBA

60S RBP L18-3

GAPDH

PTB

GAPDH

GAPDH

6

OUB2

TIP2

60S RBP L18-3

PTB

PP2A

PP2A

TUBA

PTB

7

TUBA

OUB2

PTB

TIP2

TUBA

TUBA

PTB

TIP2

8

TIP2

TUBA

TIP2

TUBA

TIP2

TIP2

TIP2

TUBA