Figure 3

DAPK2 is expressed in cortical peritubular fibroblasts. Co-immunofluorescent staining with molecular markers excluded DAPK2 expression (white arrows) from three major cell types. Proximal and distal tubules were excluded via (Figure 2A-C) lotus lectin and (A) E-cadherin staining (open arrow), (B) dendritic cells and macrophage positive for F4/80-BM8 (open arrow) did not co-localize with DAPK2, and (C) endothelial cells marked with membrane localized Meca32 (open arrow) were close to but did not co-localize with DAPK2 (inset green and red channels of dashed box area). (D) Smooth muscle cells marked by αSMA did not co-localize with DAPK2 staining (inset: cyan and red channels of dashed box area). Adjacent staining with the pericyte and fibroblast specific marker (E,E’) PDGFRβ and (F,F’) CD73 respectively, correlated positively with DAPK2 antibody labeling. Immunofluorescent images are representative of three wild type mice analyzed for each antibody. White arrows, white arrowheads, and black arrowheads correspond with equivalent positions between panels and adjacent ‘prime’ panels. Scale bars = 50 μm.