Figure 1

Expression of CYP19P1 and CYP19A1 in bovine tissues. A) Quantitative reverse transcription PCR analysis of CYP19P1 and CYP19A1 transcripts. Concentration values were normalized using RPLP0 as an internal control. The abundance of the RPLP0 transcript in the bovine tissues is indicated in the inserted diagram. The results are shown as means +/- SEM of n = 3 independent experiments. Analyzed tissues were placental cotyledons (Cot) and caruncles (Car), fetal ovaries (fOv) and granulosa cells from dominant and pre-ovulatory follicles (dGC and pGC, respectively). CYP19A1 and CYP19P1 transcripts were not detected in pGC, adrenal glands, endometria and livers. B) Agarose gels confirming the generation of only the expected products during the qPCR analysis shown in the panel A. The products of qPCR reactions including reverse transcription are indicated below the gels by white boxes labeled + RT. Control PCR reactions without prior reverse transcription (indicated by black boxes labeled –RT) did not yield products. Lanes labeled with +, - and M contain positive PCR controls (PCR products from cloned cDNAs), negative PCR controls (PCR reactions without templates) and molecular weight markers, respectively.