Figure 2

Analysis of the putative CYP19P1 promoter. The upper panel depicts a part of the bovine chromosome 10 (included in the GenBank sequence NW_003104282) with CYP19A1 and CYP19P1 depicted as arrow-headed boxes. The stippled line signifies 20 kb of intergenic DNA. Promoters (P2, P), exons (E2, E) and introns (In) are highlighted by hatching, black and white staining, respectively. Transcription start sites are represented by black arrows. The CYP19A1 GenBank sequence Z69242 (black horizontal lines below the chromosome 10 schematic) covers the proximal promoter P2, exon 2 with the translation start codon, and part of intron 2. By BLAST analysis, two homologous regions were found in the NW_003104282 sequence (shaded areas). The lower panel shows aligned sequences of homologous CYP19A1 and CYP19P1 regions. Asterisks indicate identical bases, dashes are arbitrarily inserted for optimal alignment. Transcribed sequences are printed in bold and transcription start sites are marked by arrows. The translation start codon is underlined and printed in italics. Above the CYP19A1 promoter sequence binding motifs of transcription factors GATA, SF1 and a TATA box are shown.