Time-specific metabolic changes in medium of Neospora caninum -infected and mock-infected culture of human brain microvascular endothelial cells. Temporal changes in the concentrations of pyruvate (a), ATP (b), β-hydroxybutyrate (BHB; c), total protein (d), non-esterified fatty acids (NEFA; e), and triglycerides (TGA, f) in culture medium of infected (red line) versus control (blue line). The statistical analysis was performed for every time point separately. *P < 0.05; **P < 0.01; ***P < 0.001 versus the control group, respectively. Error bars indicate the standard errors of the means (n = 6).