Table 2 Sublocalization, stability, optimal conditions and inhibition of DNase activity of S.suis P1/7

Sublocalization

  Pellets

100 ± 0.2

  Supernatant

7.2 ± 1.0

Stability

  No treatment

100 ± 0.2

  50°C/30 min

1.8 ± 0.3

  60°C/30 min

1.9 ± 1.0

  70°C/30 min

0 ± 1.8

Reactional pH

  6.0

38.5 ± 0.8

  6.5

82.1 ± 0.5

  7.0

100 ± 0.2

  7.5

98.7 ± 0.1

  8.0

96.4 ± 0.2

Incubation temperature

  25°C

10.0 ± 0.2

  30°C

49.3 ± 0.3

  37°C

100 ± 0.1

  42°C

110.6 ± 0.05

Inhibition

  None

100 ± 13.0

  EDTA 10 mM

0 ± 7.1

  EDTA 1 mM

46.8 ± 9.7

  EGTA 10 mM

0 ± 10.9

  EGTA 1 mM

0 ± 10.2

  1,10-phenanthroline 10 mM

33.3 ± 29.2

  1,10-phenanthroline 1 mM

52.4 ± 1.9