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Table 2 Sublocalization, stability, optimal conditions and inhibition of DNase activity of S.suis P1/7

From: Characterization of DNase activity and gene in Streptococcus suis and evidence for a role as virulence factor

Treatment/condition Activity (%) ± SD
Sublocalization
  Pellets 100 ± 0.2
  Supernatant 7.2 ± 1.0
Stability
  No treatment 100 ± 0.2
  50°C/30 min 1.8 ± 0.3
  60°C/30 min 1.9 ± 1.0
  70°C/30 min 0 ± 1.8
Reactional pH
  6.0 38.5 ± 0.8
  6.5 82.1 ± 0.5
  7.0 100 ± 0.2
  7.5 98.7 ± 0.1
  8.0 96.4 ± 0.2
Incubation temperature
  25°C 10.0 ± 0.2
  30°C 49.3 ± 0.3
  37°C 100 ± 0.1
  42°C 110.6 ± 0.05
Inhibition
  None 100 ± 13.0
  EDTA 10 mM 0 ± 7.1
  EDTA 1 mM 46.8 ± 9.7
  EGTA 10 mM 0 ± 10.9
  EGTA 1 mM 0 ± 10.2
  1,10-phenanthroline 10 mM 33.3 ± 29.2
  1,10-phenanthroline 1 mM 52.4 ± 1.9