Figure 4

Short-term molecular evolution of the RNA and non-RNA MTases. A – Scatter plot showing the dN and dS Log₂ values for each MTase studied in eight different patho-pangenomes. The MTases were classified according to subtract. The correlation coefficients for each type of MTases were calculated and plotted together with tendency lines. The red dashed line shows the neutrality boundary where the upper values are considered to be under positive selection and the lower one is considered to be under purifying (or stabilizing) selection. B – Boxplot showing the distribution of the (ω) omega values (Log₂). Categorization of the MTases in agreement with the plot in panel A. Deep view of the synonymous and non-synonymous substitutions on prmA from S. enterica (C) , showing one of the highest omega values, and rlmH from E. faecium (D) , showing a pattern purifying selection. These plots indicate the exact sites on proteins where the synonymous and non-synonymous substitutions predominantly lie. The critical sites for protein function are highlighted in gray.