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Table 3 Search output produced by searching E. coli flagellin MS sequence data against a curated E. coli flagellin database (CD) alone and in conjunction with the public database, Swissprot

From: Fit-for-purpose curated database application in mass spectrometry-based targeted protein identification and validation

Strain number

Confirmed serotype

MS-H type

Mascot score

Sequence identified

Sequence coverage (%)

   

CD only

CD and Swissprot

CD only

CD and Swissprot

CD only

CD and Swissprot

E169

H1

H1

14607

10922

57(55)¶

57(49)

98

98

E170

H2

H2

1754

1113

37(34)

37(27)

80

80

E171

H3

H3

8117

5735

52(46)

50(39)

91

90

E172

H4

H4

3894

2893

28(26)

28(21)

89

89

E173

H5

H5

1568

1167

26(23)

24(16)

81

74

E174

H6

H6

6123

4513

46(44)

46(38)

90

90

EDL933

H7

H7

6131

4511

56(54)

55(48)

90

90

E176

H8

H8

5538

3916

44(43)

43(39)

90

89

E177

H9

H9

10426

8099

53(51)

52(47)

80

80

E659

H10

H10

7281

5042

47(47)

47(41)

98

98

902380

H7

H7

3421

2515

43(40)

42(35)

84

82

050958

H7

H7

2656

1999

38(36)

38(31)

78

78

090414

H7

H7

5223

3943

46(44)

45(42)

94

94

091349

H7

H7

5887

4459

52(49)

52(46)

94

94

091350

H7

H7

3404

2522

44(42)

43(37)

89

88

  1. ¶Numbers without brackets denote total specific peptide match numbers while numbers in brackets denote significant specific peptide match numbers as per the Mascot search engine.