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Table 3 Search output produced by searching E. coli flagellin MS sequence data against a curated E. coli flagellin database (CD) alone and in conjunction with the public database, Swissprot

From: Fit-for-purpose curated database application in mass spectrometry-based targeted protein identification and validation

Strain number Confirmed serotype MS-H type Mascot score Sequence identified Sequence coverage (%)
    CD only CD and Swissprot CD only CD and Swissprot CD only CD and Swissprot
E169 H1 H1 14607 10922 57(55)¶ 57(49) 98 98
E170 H2 H2 1754 1113 37(34) 37(27) 80 80
E171 H3 H3 8117 5735 52(46) 50(39) 91 90
E172 H4 H4 3894 2893 28(26) 28(21) 89 89
E173 H5 H5 1568 1167 26(23) 24(16) 81 74
E174 H6 H6 6123 4513 46(44) 46(38) 90 90
EDL933 H7 H7 6131 4511 56(54) 55(48) 90 90
E176 H8 H8 5538 3916 44(43) 43(39) 90 89
E177 H9 H9 10426 8099 53(51) 52(47) 80 80
E659 H10 H10 7281 5042 47(47) 47(41) 98 98
902380 H7 H7 3421 2515 43(40) 42(35) 84 82
050958 H7 H7 2656 1999 38(36) 38(31) 78 78
090414 H7 H7 5223 3943 46(44) 45(42) 94 94
091349 H7 H7 5887 4459 52(49) 52(46) 94 94
091350 H7 H7 3404 2522 44(42) 43(37) 89 88
  1. ¶Numbers without brackets denote total specific peptide match numbers while numbers in brackets denote significant specific peptide match numbers as per the Mascot search engine.