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Table 4 Top hits produced by searching E. coli flagellin MS data against a curated E. coli flagellin database (CD) and the public databases, Swiss-prot and NCBInr a

From: Fit-for-purpose curated database application in mass spectrometry-based targeted protein identification and validation

Strain number Confirmed serotype CD (195 sequences) top hit Swiss-prot (331,337 sequen ces) top hit NCBInr (25,303,445 sequences) top hit
E169 H1 H1 Shigella flagellin flagellin [E. coli]
E170 H2 H2 E. coli Elongation factor flagellin [E. coli]
E171 H3 H3 Salmonnella flagellin flagellin [E. coli]
E172 H4 H4 E. coli K12 flagellin flagellin [E. coli]
E173 H5 H5 E. coli K12 flagellin E. coli flagellar protein FliC
E174 H6 H6 Shigella flagellin FliC [E. Coli]
EDL933 H7 H7 Shigella flagellin flagellin [E. coli]
E176 H8 H8 Shigella flagellin flagellin [E. coli]
E177 H9 H9 Shigella flagellin flagellin [E. coli]
E659 H10 H10 E. coli K12 flagellin flagellin [E. coli]
902380 H7 H7 Shigella flagellin flagellin [E. coli]
050958 H7 H7 Shigella flagellin flagellin [E. coli]
090414 H7 H7 Shigella flagellin flagellin [E. coli]
091349 H7 H7 Shigella flagellin flagellin [E. coli]
091350 H7 H7 Shigella flagellin flagellin [E. coli]
  1. aAn Orbitrap system was used with 30 ppm peptide mass tolerance, 0.5 kD MS/MS tolerance, one missed tryptic cleavage for all database searches. Oxidation on methionine and deamidation on glutamine and asparagine were chosen as a possible modification.