Identification and validation of quantitative real-time reverse transcription PCR reference genes for gene expression analysis in teak (Tectona grandis L.f.)

Table 1 Candidate reference genes, primers used to amplify in teak and their PCR parameters

Gene symbol	Gene name	Primer sequences (5′-3′) forward/reverse*	Tm (°C)	Amplicon (bp)
rp60s	Ribosomal protein 60S	ATGGTGAAGTTCTTGAAGCC/TGGTTCTTTACCAAGCTC	55	399
Cac	Clathrin adaptor complex	AAGGATAACTTTGTCATTGT/TGGGAAATACATGAAGGCG	58	794
Act	Actin	GTTAGCAATTGGGATGATATGG/ATCCAGACACTGTACTTCCT	57	797
His3	Histone 3	ACN GGTGGAGTGAAGAAGCC/TCCTTGGGCATGATN GTN AC	61	275
Sand	Sand family protein	ATATATTCCAGATATGGAGATGA/TAY ATGAAATGCCAAAGTCCA	55	941
Β-Tub	Β-Tubulin	ACN CAR CAAATGTGGGATGC/TCCCCAGTGTACCAR TGCAA	60	335
Ubq	Ubiquitin	TR ACGGGN AAGACCATAAC/ACCTTCTTN TTCTTGTGCTT	56	271
Ef-1α	Elongation factor 1- α	CATCAACATTGTGGTCATTGG/CCAGAN CGCCTGTCAATCTTG	55	1095

Degenerate bases are indicated in bold.
*Degenerate nucleotides used in some primers: N = any base, Y = C or T, R = A or G.

ISSN: 1756-0500