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Table 1 Assembly statistics of Velvet applied on Staphylococcus aureus (MRSA) strain E-MRSA15-CC22-SCC mec IV showing an increase in contig size and N50 when using higher k-mer sizes, but revealing a mis-assembly starting from k-mer size 97 using whole genome mapping

From: Employing whole genome mapping for optimal de novo assembly of bacterial genomes

K-mer size N50 Total number of contigs Longest contig size Mis-assemblies on mapped contigs* Approx. nts involved in mis-assemblies
Velvet  
81 162295 40 340060 1 (10) 122303
83 170447 38 351373 1 (9) 122303
85 170449 37 351321 0 (10)  
87 173763 33 351326 0 (10)  
89 173765 33 351394 0 (10)  
91 173767 33 351330 0 (10)  
93 173769 35 340092 0 (10)  
97 175770 33 365247 1 (9) 130273
99 175776 33 365260 1 (10) 130273
101 187438 32 365623 1 (9) 130273
103 187448 32 365625 1 (9) 130273
105 187458 32 365638 1 (9) 130273
107 187465 33 365647 1 (9) 130273
109 212189 32 365656 1 (9) 130273
111 212287 33 349286 2 (8) 93632 & 153207
113 212292 34 349288 1 (10) 93634
115 212294 34 349290 1 (10) 118928
117 174074 35 349419 1 (11) 93634
119 174076 35 349423 1 (11) 93638
121 170642 37 349435 0 (11)  
123 170654 38 340456 0 (10)  
  1. *Number of mapped contigs indicated between brackets.