Schematic diagram of ICE Sde 3396:km (not to scale). The three regions (Region 1, Region 2 and Region 3) of the ICE, and regions targeted by PCR to determine the presence of these regions (i.e. R1, R2 and R3) are shown at the top of figure. R3P depicts the location of the probe used in Southern hybridisation. The location of Nde I sites and kanamycin resistance gene (unfilled arrow) are also shown. Genes involved in mobilisation, integration into recipient chromosomes, and those involved in conferring resistance to arsenate and cadmium are shown.