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Table 2 Population genetic parameters of microsatellite loci of Garra barreimiae and cross-species amplification results

From: Characterization of 19 new microsatellite loci for the Omani barb Garra barreimiae from 454 sequences

Locus name N MF N WF Size (bp) NA HeMF HoMF Oosterhout MF HW p-value MF HeWF HoWF Oosterhout WF HW p-value WF FST CA
QLIMC 20 24 88 - 92 3 0.00 0.00 0 NV 19.42 20.83 −0.108 0 0.059 22
7GHL 20 24 166 - 250 19 89.10 90.00 −0.022 0.571 93.09 95.83 −0.027 0.777 0.025* 11
PH8AC 20 24 126 - 188 15 86.03 95.00 −0.076 0.634 91.58 75.00 0.082 0.995 0.020* 21
UHPEC 20 24 89 - 101 7 35.26 30.00 0.107 0.872 54.43 62.50 −0.087 0.437 0.071* 20
JQSOC 20 24 147 - 169 7 74.49 75.00 −0.021 0.092 75.89 62.50 0.077 0.966 0.160** 20
DL6X 20 24 112 - 126 8 60.00 65.00 −0.069 0.250 59.75 70.83 −0.120 0.273 0.156** 19
FYP9C 20 24 169 - 235 20 78.46 90.00 −0.096 0.477 95.04 87.50 0.031 0.793 0.068** 22
HOLNC 20 24 191 - 233 7 59.49 65.00 −0.067 0.128 39.89 41.67 −0.033 0.419 0.195** 22
JMLRC 20 24 93 - 99 4 57.44 65.00 −0.064 0.360 71.37 70.83 −0.007 0.818 0.120** 20
CJHGC 20 24 198 - 215 7 54.23 35.00 0.172 0.968 77.66 75.00 −0.002 0.588 0.194** 21
3Z71C 20 24 123 - 127 2 29.62 35.00 −0.194 0.000 0.00 0.00 0 NV 0.170* 22
9XNCC 20 24 101 - 112 6 71.67 70.00 −0.012 0.594 74.20 70.83 0.007 0.589 0.061* 22
3 N43 20 24 182 - 184 2 0.00 0.00 0 NV 28.37 33.33 −0.184 0 0.135* 22
1EHEC 20 24 106 - 112 4 68.08 80.00 −0.106 0.000 63.03 62.50 0.004 0.068 0.176** 22
88CMC 20 24 190 - 198 6 40.90 45.00 −0.069 0.000 73.40 54.17 0.120 0.995 0.216** 21
I6G2C 20 24 115 - 137 8 58.21 60.00 −0.012 0.684 71.45 62.50 0.048 0.781 0.282** 22
3ROZ 20 24 262 - 276 5 14.23 5.00 0.172 0.923 56.34 4.55 0.386 1.000 0.172* 0
CB75 20 24 171 - 199 7 51.41 45.00 0.067 0.767 68.17 75.00 −0.069 0.074 0.005 2
2PUMC 20 24 152 - 176 8 38.33 45.00 −0.240 0.000 77.66 87.50 −0.082 0.212 0.121** 22
  1. N number of individuals analysed, Ta (°C) annealing temperature, NA number of alleles, MF Misfat Al Abriyeen, WF Wadi Al Falahi, He expected heterozygosity in%, Ho observed heterozygosity in%, null allele estimates (Oosterhout values), HW p-value probability for deviation from Hardy-Weinberg equilibrium, FST values (*indicates significant values at p < 0.05 and **at p < 0.001); loci marked with Cwere positive and polymorphic in Garra rufa; CA number of individuals positive in cross-species amplification (tested in 22 individuals of G. rufa).