Analysis of stem cell markers expression of ADSCs from C57BL/6 J mice males and females. Results represent means ± S.D. from three independent experiments of each experimental group (days and sexes) in triplicate, obtained by flow cytometry analysis. The percentages of ADSCs mice males (A) and females (B) among 30, 90, and 120 days of age expressing hematopoietic stem cell markers (CD45 and 34), mesenchymal (CD90 and CD105), Nanog, CD106, and VEGFR-1 were correlated with the age and sex of animals. The difference between the positivity for expression of mesenchymal and hematopoietic markers was statistically significant. Statistical differences were obtained by analysis of variance ANOVA and Tukey-Kramer multiple-comparisons test. Statistical significance (p-value) *p < 0.05, **p < 0.01 and ***p < 0.001.