Figure 2From: Expression of the gene encoding oxalate decarboxylase from Bacillus subtilis and characterization of the recombinant enzymeMeasurement of enzyme activity in E. coli TOP 10 and pBy cells at pH 5, pH 6 or pH 7. E. coli TOP 10 and pBy cells were incubated with L-arabinose, MnCl2, and sodium oxalate at pH 5, pH 6, or pH 7 and the oxalate-degrading activity of OXDC in the supernatants was determined by an oxalate kit. Cells were incubated for 16 hours (A) and 24 hours (B).Back to article page