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Table 2 Log CFU mL -1 of CFS-treated L. monocytogenes (A) and S. enterica (B) co-cultures

From: Potential of cell-free supernatants from cultures of selected lactic acid bacteria and yeast obtained from local fermented foods as inhibitors of Listeria monocytogenes, Salmonella spp. and Staphylococcus aureus

A. L. monocytogenes
Day CFS (%)   CFS   
   Total LAB Lactococcus Labmix S. thermophilus Yeast sp.
0 0   4.00 × 104 ± 2.64 × 103   
3 0   1.71 × 109 ± 4.77 × 108   
  25 1.90 × 108 ± 1.13 × 108 8.00 × 107 ± 2.83 × 107 1.50 × 108 ± 1.41 × 107 3.60 × 107 ± 1.41 × 106 1.82 × 107
  50 0.0Ω 2.26 × 102 ± 3.05 × 101♦ 0.0Ω 6.25 × 104 ± 1.06 × 104 6.80 × 107 ± 2.83 × 106
B. S. enterica
Day CFS (%)   CFS   
   Total LAB Lactococcus Labmix S. thermophilus Yeast sp.
0 0   1.30 × 106 ± 1.00 × 105   
3 0   1.83 × 108 ± 1.15 × 107   
  12.5 2.50 × 102 ± 7.0 × 101 5.00 × 104 ± 0.0 4.20 × 103 ± 4.24 × 102 8.40 × 103 ± 5.65 × 102 ND
  1. Comparisons were made between CFUs from all CFS-treated co-cultures on day 3 and CFU from the control ( 0% CFS) on day 3. CFUs from the day 0 control (0% CFS) co-cultures are for reference only, i.e., to indicate both initial CFUs and that growth occurs in the absence of CFS treatment.
  2. For L. monocytogenes, this value was significantly less than its respective day 3 control CFU (P <0.05). Since all other experimental CFUs of all CFSs on day 3 were less than this value, they were also significantly less than the day 3 control CFU.
  3. ♦ - Obtained by plating 50 μL (undiluted) instead of the usual 1 μL of 10-6 or 10-4 dilutions.
  4. Ω - This value was zero even after plating 50 μL direct from culture instead of 1 μL of dilutions.
  5. For S. enterica, this value was significantly less than its respective day 3 control CFU (P <0.05). Since all other experimental CFUs of all CFSs on day 3 were less than this value, they were also significantly less than the day 3 control CFU.
  6. ND – Not done.