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Table 3 Log CFU mL -1 of CFS-treated Salmonella (MI1) (A) and S. aureus (B) co-cultures

From: Potential of cell-free supernatants from cultures of selected lactic acid bacteria and yeast obtained from local fermented foods as inhibitors of Listeria monocytogenes, Salmonella spp. and Staphylococcus aureus

A. Salmonella MI1

Day

CFS (%)

 

CFS

  
  

Total LAB

Lactococcus

Labmix

S. thermophilus

Yeast sp.

0

0

 

7.05 × 104 ± 2.64 × 103

  

3

0

 

2.06 × 109 ± 1.58 × 108

  
 

12.5

1.90 × 107 ± 1.41 × 10

9.90 × 107 ± 1.41 × 106

3.06 × 104 ± 8.48 × 102*

3.05 × 105 ± 7.07 × 103

2.10 × 108 ± 2.82 × 107∞

 

25

0.0

3.20 × 104*

0.0**

3.70 × 102 ± 9.8 × 101

2.51 × 107 ± 1.41 × 105

B. S. aureus

Day

CFS (%)

 

CFS

  

C.

 

Total LAB

Lactococcus

Labmix

S. thermophilus

Yeast sp.

0

0

 

6.27 × 104 ± 6.43 × 103

  

3

0

 

1.05 × 109 ± 2.12 × 108

  
 

12.5

9.30 × 106 ± 1.27 × 106

6.00 × 107 ± 4.24 × 10

5.15 × 105 ± 2.12 × 104

1.95 × 107 ± 1.41 × 106

5.40 × 107 ± 2.82 × 106

 

25

6.80 × 104 ± 1.69 × 104

8.50 × 105 ± 4.94 × 105

1.09 × 105 ± 7.07 × 103

0.0

2.37 × 107 ± 2.40 × 106

  1. Comparisons were made between CFUs from all CFS-treated co-cultures on day 3 and CFU from the control on day 3. CFUs from the day 0 control (0% CFS) co-cultures are for reference only, i.e., to indicate both initial CFUs and that growth occurs in the absence of CFS treatment.
  2. Δ - Colonies were extremely small even after prolonged incubation for 48 hrs.
  3. * - Obtained by plating of 50 μL (undiluted) instead of the previous 1 μL (undiluted) plating.
  4. ** - This value was 0.0 even after plating 10 or 50 μL directly (without dilution) instead of 1 μL.
  5. For Salmonella (MI1), this value was significantly less than its respective day 3 control CFU (P ≤ 0.05). Since all other experimental CFUs of all CFSs on day 3 were less than this value, they were also significantly less than the day 3 CFU.
  6. θFor S. aureus, this value was significantly less than its respective day 3 control CFU (P ≤ 0.05). Since all other experimental CFUs of all CFSs on day 3 were less than this value, they were also significantly less than the day 3 CFU.