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Table 3 Log CFU mL -1 of CFS-treated Salmonella (MI1) (A) and S. aureus (B) co-cultures

From: Potential of cell-free supernatants from cultures of selected lactic acid bacteria and yeast obtained from local fermented foods as inhibitors of Listeria monocytogenes, Salmonella spp. and Staphylococcus aureus

A. Salmonella MI1
Day CFS (%)   CFS   
   Total LAB Lactococcus Labmix S. thermophilus Yeast sp.
0 0   7.05 × 104 ± 2.64 × 103   
3 0   2.06 × 109 ± 1.58 × 108   
  12.5 1.90 × 107 ± 1.41 × 10 9.90 × 107 ± 1.41 × 106 3.06 × 104 ± 8.48 × 102* 3.05 × 105 ± 7.07 × 103 2.10 × 108 ± 2.82 × 107∞
  25 0.0 3.20 × 104* 0.0** 3.70 × 102 ± 9.8 × 101 2.51 × 107 ± 1.41 × 105
B. S. aureus
Day CFS (%)   CFS   
C.   Total LAB Lactococcus Labmix S. thermophilus Yeast sp.
0 0   6.27 × 104 ± 6.43 × 103   
3 0   1.05 × 109 ± 2.12 × 108   
  12.5 9.30 × 106 ± 1.27 × 106 6.00 × 107 ± 4.24 × 10 5.15 × 105 ± 2.12 × 104 1.95 × 107 ± 1.41 × 106 5.40 × 107 ± 2.82 × 106
  25 6.80 × 104 ± 1.69 × 104 8.50 × 105 ± 4.94 × 105 1.09 × 105 ± 7.07 × 103 0.0 2.37 × 107 ± 2.40 × 106
  1. Comparisons were made between CFUs from all CFS-treated co-cultures on day 3 and CFU from the control on day 3. CFUs from the day 0 control (0% CFS) co-cultures are for reference only, i.e., to indicate both initial CFUs and that growth occurs in the absence of CFS treatment.
  2. Δ - Colonies were extremely small even after prolonged incubation for 48 hrs.
  3. * - Obtained by plating of 50 μL (undiluted) instead of the previous 1 μL (undiluted) plating.
  4. ** - This value was 0.0 even after plating 10 or 50 μL directly (without dilution) instead of 1 μL.
  5. For Salmonella (MI1), this value was significantly less than its respective day 3 control CFU (P ≤ 0.05). Since all other experimental CFUs of all CFSs on day 3 were less than this value, they were also significantly less than the day 3 CFU.
  6. θFor S. aureus, this value was significantly less than its respective day 3 control CFU (P ≤ 0.05). Since all other experimental CFUs of all CFSs on day 3 were less than this value, they were also significantly less than the day 3 CFU.