Calpain-5 is stably expressed in HEK293T cells. HEK293T cells were co-transfected with CAPN5-p.R243L and puromycin plasmids and twenty-two clones were analyzed. Calpain-5 protein expression was detected with an anti-myc antibody in protein extracts (upper panels). GAPDH levels are indicated (lower panels). A HEK293T cell line that showed moderate expression (lane 16) was propagated for use in the silencing experiments.