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Figure 3 | BMC Research Notes

Figure 3

From: Evaluation of pre-induction temperature, cell growth at induction and IPTG concentration on the expression of a leptospiral protein in E. coli using shaking flasks and microbioreactor

Figure 3

SDS-PAGE with uninduced and IPTG-induced E. coli BL21 (DE3) Star/pAE/LigB (131-645aa) samples normalized by absorbance at 600 nm from assay 3 (Abs ind = 2.0 and IPTG 0.1 mM), total protein extract separated into soluble and insoluble fractions of recombinant protein in 20 mM Tris/1 mM EDTA/0.1% Triton X-100 (pH8). Unind = uninduced; Ind = induced; TE = Total Extract.

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