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Table 3 Statistics of incremental bark assemblies across k-mers

From: RNA sequencing read depth requirement for optimal transcriptome coverage in Hevea brasiliensis

k-mer size 1 Gb 3 Gb 5 Gb 8 Gb 10 Gb 13 Gb 16 Gb
N50 (bp) Total transcripts N50 (bp) Total transcripts N50 (bp) Total transcripts N50 (bp) Total transcripts N50 (bp) Total transcripts N50 (bp) Total transcripts N50 (bp) Total transcripts
51 1,389 68,942 1,734 102,352 1,741 131,979 1,695 170,838 1,648 193,885 1,599 224,292 1,542 254,026
53 1,375 64,265 1,763 94,288 1,797 120,998 1,778 157,127 1,741 178,204 1,701 206,355 1,654 234,717
55 1,353 59,325 1,783 86,117 1,832 110,086 1,834 142,292 1,813 161,486 1,798 186,542 1,758 212,283
57 1,343 54,670 1,798 78,651 1,852 100,131 1,872 129,469 1,864 146,550 1,861 169,917 1,828 192,775
59 1,315 50,440 1,799 72,051 1,876 91,351 1,905 117,384 1,901 133,175 1,903 154,642 1,880 176,018
61 1,288 46,261 1,804 65,903 1,891 83,027 1,926 106,569 1,933 120,901 1,940 139,498 1,928 159,832
63 1,255 42,626 1,791 60,666 1,889 75,893 1,959 96,676 1,959 109,522 1,969 126,478 1,956 143,857
65 1,225 39,223 1,782 55,645 1,900 69,159 1,966 87,780 1,980 98,782 1,989 115,028 1,988 130,546
67 1,199 35,773 1,753 51,333 1,892 63,469 1,970 80,153 1,997 90,055 2,010 104,399 2,025 118,385
69 1,147 32,606 1,731 47,383 1,895 58,112 1,976 72,612 2,007 81,560 2,024 94,500 2,043 106,974
71 1,111 29,621 1,691 43,910 1,878 53,357 1,974 66,249 2,017 74,220 2,036 85,874 2,061 96,653
73 1,068 26,674 1,652 40,633 1,846 49,412 1,967 60,561 2,010 67,593 2,040 77,667 2,068 87,612
75 1,034 23,699 1,606 37,433 1,808 45,312 1,946 55,384 1,997 61,680 2,041 70,331 2,066 79,038
77 982 20,746 1,550 34,531 1,770 41,621 1,923 50,636 1,967 56,233 2,022 63,767 2,057 71,504
  1. The peak N50 length from each datasize assembly (other than for 1 Gb) is highlighted in bold italics.