Figure 3From: Molecular cloning and in-silico characterization of high temperature stress responsive pAPX gene isolated from heat tolerant Indian wheat cv. Raj 3765Heat stress study of recombinant E. coli BL21 (pET28a- TapAPX ) cells. OD reading of E. coli BL21 (pET28) cells and E. coli BL21 (pET28-TapAPX) cells grown at different temperatures after IPTG induction (A). SDS-PAGE analysis of total protein (10 μg) of E. coli BL21 (pET28) cells and E. coli BL21 (pET28-T apAPX) cells subjected to heat stress, M-Marker (B). *indicates significant difference as determined by simple pair wise t-test comparison (α = 0.05).Back to article page