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Figure 3 | BMC Research Notes

Figure 3

From: The cytological and molecular role of DOMAINS REARRANGED METHYLTRANSFERASE3 in RNA-dependent DNA methylation of Arabidopsis thaliana

Figure 3

DRM3 colocalizes with AGO4 in a nuclear domain in the nucleolar periphery. (A) Methylation Sensitive PCR showing that the 35S:YFP-DRM3 transgene rescues the methylation phenotype of drm3. (B) Confocal microscope inspection showing transgene expression throughout the root (left panel) and the preferential accumulation of 35S:YFP-DRM3 in a round focus in the nucleolar periphery (right panel). (C) Confocal projections of root meristem nuclei indicate that interphase distribution of DRM3 does not depend on Pol V, AGO4 or DRM2 activity. (D) Immunolocalization of AGO4 (red) and DRM3 (green) show colocalization of both proteins in the smRNA perinucleolar body (>98%; n = 85). DNA counterstained with DAPI (blue). -500 bp.

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