Figure 2

Effect of SINV infection on hNPCs proliferation and undifferentiated phenotype. hNPCs were mock infected or infected with SINV at m.o.i 1 (A, B) Cell proliferation was analyzed by an EdU incorporation assay. A representative blot is shown in (A), samples were gated on EdU staining positive cells, and percentage of EdU incorporated cells in SINV infected sample were shown. (B) Percentage of EdU incorporated cells in mock and SINV infected cells were quantified by flow cytometry. Error bars indicate SDs. *, statistical significance (p < 0.05) in comparison with mock. (C) At 48 hour after infection, expression of apoptotic cell marker active caspase 3 was analyzed by flow cytometry. This experiment was repeated at least twice, two titrations per experiment. Error bars indicate SDs. *, statistical significance (p < 0.05) compared to the mock infected control at same time point. (D, E) Western Blot analysis of the expression of active caspase 3 and proliferating cell marker PCNA during the infection time course. Each experiment was performed at least three times. A representative blot is shown in (D). Blots were scanned, and relative expression levels of proteins were normalized to GAPDH and shown in (E). Error bars indicated SDs. *, statistical significance (p < 0.05) compared to the mock.