Figure 2

Western blot analysis showing the immunogenicity and specificity of the purified UreG recombinant protein. A: rUreG purified under native condition probed with monoclonal anti-histidine-HRP B: rUreG purified under denaturing condition probed with monoclonal anti-histidine-HRP C: rUreG incubated with primary antibodies from different groups of serum samples: 1: Primary antibody from the Group I serum sample (culture-positive patient) 2: Primary antibody from the Group II serum sample (healthy person) 3: Primary antibody from the Group III serum sample (culture-negative patients seronegative) 4: Primary antibody from the Group IV serum sample (patient with other disease) MW: low molecular weight marker.