Figure 2

Map of six SNPs and EMSA for rs1800734. a. Map of six SNPs, rs2276807 (C/A), rs4678922 (A/T), rs6789043 (T/C), rs1046512 (C/A), rs3774343 (G/A) and rs4647215 (C/A), located in the 54-kb region surrounding the MLH1-93G/A SNP. b. EMSA using nuclear extracts from HeLa cells. The competition assay was performed with ³²P-labeled MLH1-184 to -132 and unlabeled MLH1-184 to -132 oligomer (lanes 1–3). A shifted band was observed in the presence of HeLa nuclear extract, which was blocked in the presence of excess unlabeled competitor (lanes 2 and 3). The competition assay was performed with matched sets of DNA oligomers homologous to each genotype of the MLH1-93G/A SNP (lanes 5–7, 9–11). When a ³²P-labeled MLH-93G probe was mixed with nuclear extract from HeLa cells, a shifted band was blocked by the competitor oligomer of MLH1-93G, but not MLH1-A (lanes 5–7). When ³²P-labeled MLH-93A probe was mixed with the nuclear extract from HeLa cells, a shifted band was not detected (lanes 9–11).