Figure 1From: Development and assessment of multiplex high resolution melting assay as a tool for rapid single-tube identification of five Brucella speciesMelt peaks of one HRM assay with titrations of target and non target DNA. The results of a titration range of 1Â ng-100Â fg genomic DNA from B. melitensis 16Â M (red) and B. abortus 544 (blue) using the B. melitensis HRM assay and the negative first derivative melt peak data. As shown, whilst the peak size does vary with the concentration of DNA, there is clear differentiation between the two species.Back to article page