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Figure 5 | BMC Research Notes

Figure 5

From: The dilution effect and the importance of selecting the right internal control genes for RT-qPCR: a paradigmatic approach in fetal sheep

Figure 5

Pattern of total RNA and raw RT-qPCR data of ICGs and rRNA through gestation. A) Total RNA concentration (μg/μg tissue) and geometrical average expression of raw RT-qPCR data of all ICGs at four time points during gestation; samples included: 50dG n = 30, 100dG n = 28, 125dG n = 25 and 140dG n = 23. B) Pattern of raw RT-qPCR data of RNA18S and RNA28S during gestation; samples included: 50dG n = 30, 100dG n = 28, 125dG n = 25 and 140dG n = 23. Kruskal-Wallis One Way Analysis of Variance on Ranks (followed by Dunn's test): different letters indicate significant (p < 0.05) differences of ICGs and RNA18S and different numbers indicate significance differences in the RNA concentration and RNA28S across gestation.

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