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Figure 1 | BMC Research Notes

Figure 1

From: Four Core Genotypes mouse model: localization of the Sry transgene and bioassay for testicular hormone levels

Figure 1

Location of the Sry transgene in four core genotypes mouse model. Samples were from XX and XY mice with testes (XXSry, XY Sry) and XX and XY mice with ovaries (XX, XY). Confirmation of transgene-genome boundary by PCR using transgene-specific primer d and Chr3 primer e (A) , with transgene-specific primer c and Chr3 primer b (B), and with transgene-specific primer c and Chr3 primer a (C). (D) The concatemer of Sry transgene is inserted into a repetitive sequence present genome-wide. (E) Sry transgene location on chromosome 3. (F) A visual estimate of the difference in copy number of Sry between wildtype and XY Sry genomic DNA in agarose gel.

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