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Figure 1 | BMC Research Notes

Figure 1

From: Four Core Genotypes mouse model: localization of the Sry transgene and bioassay for testicular hormone levels

Figure 1

Location of the Sry transgene in four core genotypes mouse model. Samples were from XX and XY mice with testes (XXSry, XY− Sry) and XX and XY mice with ovaries (XX, XY−). Confirmation of transgene-genome boundary by PCR using transgene-specific primer d and Chr3 primer e (A) , with transgene-specific primer c and Chr3 primer b (B), and with transgene-specific primer c and Chr3 primer a (C). (D) The concatemer of Sry transgene is inserted into a repetitive sequence present genome-wide. (E) Sry transgene location on chromosome 3. (F) A visual estimate of the difference in copy number of Sry between wildtype and XY− Sry genomic DNA in agarose gel.

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BMC Research Notes

ISSN: 1756-0500

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