Location of the
transgene in four core genotypes mouse model. Samples were from XX and XY mice with testes (XXSry, XY−
Sry) and XX and XY mice with ovaries (XX, XY−). Confirmation of transgene-genome boundary by PCR using transgene-specific primer d and Chr3 primer e
, with transgene-specific primer c and Chr3 primer b
(B), and with transgene-specific primer c and Chr3 primer a
(C). (D) The concatemer of Sry transgene is inserted into a repetitive sequence present genome-wide. (E)
Sry transgene location on chromosome 3. (F) A visual estimate of the difference in copy number of Sry between wildtype and XY−
Sry genomic DNA in agarose gel.