F]FDG distribution after simulated herbivory in
plants. (A) Autoradiograph from plant parts of [18F]FDG-treated wild type N. attenuata plants. Leaves of 3.5 week old plants were punctured with a needle and applied with [18F]FDG. Another leaf kept untreated (CON) or was wounded and treated with 1 μL of water (WW) or 1 μL of 1:5 diluted Manduca sexta oral secretions (WOS). After 8 hours the plants were disassembled and an autoradiographic picture was taken. (B) Root pictures from (A) were magnified and assembled next to each other to demonstrate the reduced accumulations of radioactivity after WOS treatments. (C) Autoradiographed roots of [18F]FDG-treated N. attenuata plants. Leaves of three week old plants were punctured with a needle and applied with [18F]FDG. Another leaf was wounded and treated with 1 μL of water (WW) or 1 μL of the fatty acid-amino acid conjugate N-linolenoyl-glutamate (WFAC), one of the active elicitors in M. sexta oral secretions.