Figure 1
From: In vitro application of ribonucleases: comparison of the effects on mRNA and miRNA stability
The Stability of ribosomal RNA is affected in a treatment-dependent manner. (A) Representative electropherograms of untreated RNA (black) and RNA after treatment with either Benzonase (dark orange), NaOH (pink), RNase A (red), RNase H (grey), RNase If (bright orange), Exonuclease T7 (dark blue) or Exonuclease T (bright blue). Electropherogram of RNA ladder, used for size calculation indicated in the abscissa as kilobases (kb), is illustrated in green. Arbitrary units for fluorescence are given in the ordinate. The untreated control sample represents four peaks: the loading marker at 25 nt, the spiked tRNA at about 80 nt, the 18S and the 28S ribosomal RNA. (B) Detection of 18S ribosomal RNA using RT-qPCR. Percentage of intact 18S ribosomal RNA after indicated treatments is illustrated for three independent experiments with mean and SD (in red). The relative percentage of intact RNA in comparison to the untreated RNA is given in the ordinate. The green dotted line represents the 100% intact 18S ribosomal RNA obtained from the untreated RNA. Significances were calculated by the two tailed unpaired Student’s t-test: * = p < 0.05; *** = p < 0.001. ExoT7 = Exonuclease T7; ExoT = Exonuclease T.