Table 1 Ribonucleases/NaOH treatments with the concentration used in this study
From: In vitro application of ribonucleases: comparison of the effects on mRNA and miRNA stability
Treatment | Units used per ml | Supplier | Enzyme commission number | Literature |
|---|---|---|---|---|
RNase A | 3.13 | Applichem, Darmstadt, Germany | EC3.1.27.5 | |
RNase H | 12.50 | Ambion, Kassel, Germany | EC3.1.26.4 | [38] |
Benzonase | 8.33 | Sigma, Steinheim, Germany | EC3.4.23.1 | [39] |
Exonuclease T | 12.50 | New England Biolabs, Frankfurt, Germany | EC3.1.3.1 | |
Exonuclease T7 | 12.50 | New England Biolabs | EC3.1.11.3 | [34] |
RNase If | 12.50 | New England Biolabs | EC3.1.27.6 | |
NaOH | 100 μmol | Applichem |  |  |