Figure 3

Tissue expression of gilthead sea bream cathepsin D paralogues. (A) Qualitative transcript expression profiles of SaCTSDa and SaCTSDb across adult gilthead sea bream (Sa) tissue types: fast skeletal muscle (FM), slow skeletal muscle (SM), heart (HE), brain (BR), liver (LI) spleen (SP) and immature gonad (GO). SaEF1α was also amplified as a reference gene to confirm a steady-state level of expression among tissues. No template (NTC) and no reverse transcriptase (RTC) negative controls were also included to confirm primer specificity and the absence of genomic DNA. Representative image and semi-quantification of band intensity from n = 3 fish. (B) Quantitative relative expression normalized to Saβ-actin of SaCTSDa and SaCTSDb from gilthead sea bream (Sa) fast skeletal muscle (left) and gonad (right). Results are shown as mean ± SEM (n = 4-6). The asterisk indicates significant differences at p < 0.05.