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Table 2 Quantification of genomic DNA extracted from pig tissue and soil microbiomes

From: Optimizing a PCR protocol for cpn60-based microbiome profiling of samples variously contaminated with host genomic DNA

Sample Gene copies per 20 ng DNAa
Pig α-actin Bacterial 16S rRNA Pig:bacteria DNA ratio
Brain 9.57 × 105 ± 2.75 × 105 1.20 × 104 ± 1.79 × 103 80:1
Nasal cavity 5.65 × 106 ± 7.07 × 104 6.77 × 106 ± 6.91 × 105 0.8:1
Stomach mucosa 4.76 × 106 ± 1.23 × 106 2.04 × 106 ± 1.26 × 105 2.3:1
Colon mucosa 8.21 × 106 ± 2.58 × 103 6.08 × 106 ± 3.30 × 104 1.3:1
Feces 1.09 × 105 ± 6.21 × 103 2.45 × 108 ± 4.79 × 107 0.0004:1
Soil Not detected 3.81 × 105 ± 2.82 × 105 All bacterial
  1. aQuantities reported are the average of duplicates ± standard deviation.