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Table 2 Quantification of genomic DNA extracted from pig tissue and soil microbiomes

From: Optimizing a PCR protocol for cpn60-based microbiome profiling of samples variously contaminated with host genomic DNA

Sample

Gene copies per 20 ng DNAa

Pig α-actin

Bacterial 16S rRNA

Pig:bacteria DNA ratio

Brain

9.57 × 105 ± 2.75 × 105

1.20 × 104 ± 1.79 × 103

80:1

Nasal cavity

5.65 × 106 ± 7.07 × 104

6.77 × 106 ± 6.91 × 105

0.8:1

Stomach mucosa

4.76 × 106 ± 1.23 × 106

2.04 × 106 ± 1.26 × 105

2.3:1

Colon mucosa

8.21 × 106 ± 2.58 × 103

6.08 × 106 ± 3.30 × 104

1.3:1

Feces

1.09 × 105 ± 6.21 × 103

2.45 × 108 ± 4.79 × 107

0.0004:1

Soil

Not detected

3.81 × 105 ± 2.82 × 105

All bacterial

  1. aQuantities reported are the average of duplicates ± standard deviation.